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superscript® iv reverse transcriptase cdna synthesis kit  (Thermo Fisher)


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    Thermo Fisher superscript® iv reverse transcriptase cdna synthesis kit
    Superscript® Iv Reverse Transcriptase Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iv+reverse+transcriptase+cdna+synthesis+kit/pm38253274-121-10-17
    Average 90 stars, based on 1 article reviews
    superscript® iv reverse transcriptase cdna synthesis kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Multiple Displacement Amplification:

    Article Title: IQGAP2 acts as a tumor suppressor in breast cancer and its reduced expression promotes cancer growth and metastasis by MEK/ERK signalling pathways
    Article Snippet: Hereafter these cells will be referred as MCF7 IQGAP2_Ex (MCF7 with IQGAP2 ectopic expression), MCF7 Control_EV (MCF7 with empty expression vector), MCF7 IQGAP2_KD (MCF7 with IQGAP2 knock down), MCF7 Control_Sc (MCF7 having knock down vector with scrambled sequence), MDA-MB-468 IQGAP2_KD (MDA-MB-468 with IQGAP2 knock down), MDA-MB-468 Control_Sc (MDA-MB-468 having knock down vector with scrambled sequence). .. To quantify the endogenous RNA level of IQGAP2, RNeasy Mini Kit (Qiagen) was used to isolate total RNA from different breast cancer cell lines i.e. MCF7, T-47D, MDA-MB-453, MDA-MB-468, MCF 10A and MDA-MB-231 as per the manufacturer’s protocol. cDNA was synthesised using Superscript IV Reverse Transcriptase cDNA synthesis kit (Invitrogen). ..

    Reverse Transcription:

    Article Title: IQGAP2 acts as a tumor suppressor in breast cancer and its reduced expression promotes cancer growth and metastasis by MEK/ERK signalling pathways
    Article Snippet: Hereafter these cells will be referred as MCF7 IQGAP2_Ex (MCF7 with IQGAP2 ectopic expression), MCF7 Control_EV (MCF7 with empty expression vector), MCF7 IQGAP2_KD (MCF7 with IQGAP2 knock down), MCF7 Control_Sc (MCF7 having knock down vector with scrambled sequence), MDA-MB-468 IQGAP2_KD (MDA-MB-468 with IQGAP2 knock down), MDA-MB-468 Control_Sc (MDA-MB-468 having knock down vector with scrambled sequence). .. To quantify the endogenous RNA level of IQGAP2, RNeasy Mini Kit (Qiagen) was used to isolate total RNA from different breast cancer cell lines i.e. MCF7, T-47D, MDA-MB-453, MDA-MB-468, MCF 10A and MDA-MB-231 as per the manufacturer’s protocol. cDNA was synthesised using Superscript IV Reverse Transcriptase cDNA synthesis kit (Invitrogen). ..

    Article Title: Single Cell transcriptional analysis of ex vivo models of cortical and hippocampal development identifies unique longitudinal trends
    Article Snippet: .. RNA from cortex and hippocampus derived ex vivo cultures was extracted from 3 biological replicates for three time points (DIV3, DIV15, DIV31) using RNeasy Plus Mini Kit (Qiagen). cDNA was synthesized using a SuperScript IV Reverse Transcriptase cDNA synthesis kit (Invitrogen) using 250ng per sample. .. Quantitative real-time PCR was performed on a QuantStudio 5 (Applied Biosystems) using TaqMan Universal PCR Master Mix (Applied Biosystems) with two technical replicates per reaction.

    Article Title: Horizontal Gene Transfer and Tandem Duplication Shape the Unique CAZyme Complement of the Mycoparasitic Oomycetes Pythium oligandrum and Pythium periplocum
    Article Snippet: .. cDNA synthesis was carried out using the Superscript IV Reverse transcriptase cDNA synthesis kit (Thermo Fisher Scientific) using 2 μg template RNA. ..

    Article Title: Pathogen-Mediated Stomatal Opening: A Previously Overlooked Pathogenicity Strategy in the Oomycete Pathogen Phytophthora infestans
    Article Snippet: RNA samples were assessed for purity and integrity by agarose gel electrophoresis and Nanodrop Spectrophotometry. .. First strand cDNA was synthesized from 20 g total RNA by oligo(dT) priming using the Superscript IV Reverse transcriptase cDNA synthesis kit (Thermo Scientific). ..

    cDNA Synthesis:

    Article Title: IQGAP2 acts as a tumor suppressor in breast cancer and its reduced expression promotes cancer growth and metastasis by MEK/ERK signalling pathways
    Article Snippet: Hereafter these cells will be referred as MCF7 IQGAP2_Ex (MCF7 with IQGAP2 ectopic expression), MCF7 Control_EV (MCF7 with empty expression vector), MCF7 IQGAP2_KD (MCF7 with IQGAP2 knock down), MCF7 Control_Sc (MCF7 having knock down vector with scrambled sequence), MDA-MB-468 IQGAP2_KD (MDA-MB-468 with IQGAP2 knock down), MDA-MB-468 Control_Sc (MDA-MB-468 having knock down vector with scrambled sequence). .. To quantify the endogenous RNA level of IQGAP2, RNeasy Mini Kit (Qiagen) was used to isolate total RNA from different breast cancer cell lines i.e. MCF7, T-47D, MDA-MB-453, MDA-MB-468, MCF 10A and MDA-MB-231 as per the manufacturer’s protocol. cDNA was synthesised using Superscript IV Reverse Transcriptase cDNA synthesis kit (Invitrogen). ..

    Article Title: Single Cell transcriptional analysis of ex vivo models of cortical and hippocampal development identifies unique longitudinal trends
    Article Snippet: .. RNA from cortex and hippocampus derived ex vivo cultures was extracted from 3 biological replicates for three time points (DIV3, DIV15, DIV31) using RNeasy Plus Mini Kit (Qiagen). cDNA was synthesized using a SuperScript IV Reverse Transcriptase cDNA synthesis kit (Invitrogen) using 250ng per sample. .. Quantitative real-time PCR was performed on a QuantStudio 5 (Applied Biosystems) using TaqMan Universal PCR Master Mix (Applied Biosystems) with two technical replicates per reaction.

    Article Title: Horizontal Gene Transfer and Tandem Duplication Shape the Unique CAZyme Complement of the Mycoparasitic Oomycetes Pythium oligandrum and Pythium periplocum
    Article Snippet: .. cDNA synthesis was carried out using the Superscript IV Reverse transcriptase cDNA synthesis kit (Thermo Fisher Scientific) using 2 μg template RNA. ..

    Article Title: Pathogen-Mediated Stomatal Opening: A Previously Overlooked Pathogenicity Strategy in the Oomycete Pathogen Phytophthora infestans
    Article Snippet: RNA samples were assessed for purity and integrity by agarose gel electrophoresis and Nanodrop Spectrophotometry. .. First strand cDNA was synthesized from 20 g total RNA by oligo(dT) priming using the Superscript IV Reverse transcriptase cDNA synthesis kit (Thermo Scientific). ..

    Derivative Assay:

    Article Title: Single Cell transcriptional analysis of ex vivo models of cortical and hippocampal development identifies unique longitudinal trends
    Article Snippet: .. RNA from cortex and hippocampus derived ex vivo cultures was extracted from 3 biological replicates for three time points (DIV3, DIV15, DIV31) using RNeasy Plus Mini Kit (Qiagen). cDNA was synthesized using a SuperScript IV Reverse Transcriptase cDNA synthesis kit (Invitrogen) using 250ng per sample. .. Quantitative real-time PCR was performed on a QuantStudio 5 (Applied Biosystems) using TaqMan Universal PCR Master Mix (Applied Biosystems) with two technical replicates per reaction.

    Ex Vivo:

    Article Title: Single Cell transcriptional analysis of ex vivo models of cortical and hippocampal development identifies unique longitudinal trends
    Article Snippet: .. RNA from cortex and hippocampus derived ex vivo cultures was extracted from 3 biological replicates for three time points (DIV3, DIV15, DIV31) using RNeasy Plus Mini Kit (Qiagen). cDNA was synthesized using a SuperScript IV Reverse Transcriptase cDNA synthesis kit (Invitrogen) using 250ng per sample. .. Quantitative real-time PCR was performed on a QuantStudio 5 (Applied Biosystems) using TaqMan Universal PCR Master Mix (Applied Biosystems) with two technical replicates per reaction.

    Synthesized:

    Article Title: Single Cell transcriptional analysis of ex vivo models of cortical and hippocampal development identifies unique longitudinal trends
    Article Snippet: .. RNA from cortex and hippocampus derived ex vivo cultures was extracted from 3 biological replicates for three time points (DIV3, DIV15, DIV31) using RNeasy Plus Mini Kit (Qiagen). cDNA was synthesized using a SuperScript IV Reverse Transcriptase cDNA synthesis kit (Invitrogen) using 250ng per sample. .. Quantitative real-time PCR was performed on a QuantStudio 5 (Applied Biosystems) using TaqMan Universal PCR Master Mix (Applied Biosystems) with two technical replicates per reaction.

    Article Title: Pathogen-Mediated Stomatal Opening: A Previously Overlooked Pathogenicity Strategy in the Oomycete Pathogen Phytophthora infestans
    Article Snippet: RNA samples were assessed for purity and integrity by agarose gel electrophoresis and Nanodrop Spectrophotometry. .. First strand cDNA was synthesized from 20 g total RNA by oligo(dT) priming using the Superscript IV Reverse transcriptase cDNA synthesis kit (Thermo Scientific). ..

    other:

    Article Title: SMARCA4 Depletion Induces Cisplatin Resistance by Activating YAP1-Mediated Epithelial-to-Mesenchymal Transition in Triple-Negative Breast Cancer
    Article Snippet: Total RNA was isolated from MDA-MB-468 and MDA-MB-231 cells with RNeasy mini kit (74106; Qiagen, Hilden, Germany) according to the manufacturer’s instructions, and 1 μg RNA was converted to cDNA using the SuperScriptTM IV Reverse Transcriptase cDNA Synthesis Kit (18090050; Invitrogen, Carlsbad, CA, USA).



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